Histological study of preventive role of cyanacobalamin [vitamin B-12] on heat induced testicular injury

The purpose of this study was to investigate the possible role of Cyanacobalamin [Vitamin B-12] in reducing the hazardous effects of heat on seminiferous tubules of testes in albino rats. Experimental Study. This study was conducted in the Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, for 6 weeks from October 2010 to November 2010. Thirty adult albino rats of 200-250 grams of weight and 90-120 days of age were taken for this study. They were divided into three groups A [control], B [heat treated], and C [heat plus Cyanocobalamin treated]. They were further subdivided into A1 and A2, B1 and B2 and C1 and C2, based on duration of treatment of 4 weeks and 6 weeks respectfully. At the end of study histological examination of seminiferous tubules of testes were seen by applying Periodic Acid Schiff Iron Hematoxylin stain. There was marked damaging effects of heat [42°C] on seminiferous tubules of testes with disorganized germinal epithelium and vacuolation. This damage to spermatogenic cell series was well protected with concomitant treatment with Cyanacobalamin [vitamin B-12]. There was restoration of germinal epithelium and marked decrease in vacuolation. This study proved protective role of Cyanacobalmin [Vitamin B-12] in heat induced damage in testes of albino rats

Morphometric study of ketoconazole treated liver in albino rats

To study the effect of ketoconazole induced liver damage, compare with control group and correlate with previous studies. Experimental study conducted during year 2005 in the Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre,Karachi. Forty adult male albino rates were used for this study. Group-A served as control animals, received injection of normal saline in dose of 0.05 ml/100 gm of body weight inraperitoneally daily for 03, 07, 05 and 30 days. Group-B received injection of ketoconazole 40 mg/kg of body weigh intraperitoneally daily for 03, 07, 15 and 30 days of treatment. Animals were sacrificed after completion of treatment under ether anaesthesia. Liver were removed, fixed in 10% and alcoholic formalin for 24-48 hours. They were dehydrated in ascending strength of alcohol and paraffin tissue blocks were made 5 microm thick section were stained with HandE for general morphology and micrometry and the result were compared with control. Analyzed satistically with student 't' test and correlate with previous studies. Ketoconazole treated animals showed distortion of hepatic architecture increase size of hepatocytes, decrease nuclear diameter and necrosis of hepatocytes within hepatic lobule as compared to control group-A animals. It was sconcluded from this study that ketoconazole induced injury is dose and duration of therapy dependent and due to its cost effective frequent use needs further research in humans

Preventive role of fresh garlic on high-fat diet induced fatty liver in albino rats

Obesity has become a worldwide problem due to westernization of diet, even in developing countries leading to consumption of high-fat diet. One of the most common diseases resulting from use of high-fat diet is fatty liver. Garlic is one of the oldest herbs used for medicinal purposes since ancient times. So the present study was undertaken to observe the protective role of fresh garlic on high-fat diet induced fatty liver in albino rats and correlate the results with previous studies. Thirty adult albino rats, weighing from 200-240 gram were taken for this study. The rats were divided into 3 groups according to dietary regimen, Group A received control diet, Group B received high- fat diet [20 ml corn oil in 100 gm of diet] and Group C received high-unsaturated fat diet with fresh garlic [20 ml corn oil with 6 gm fresh garlic in 100 gm of diet], for 8 weeks at the end of which they were sacrificed and tissue slides stained with haematoxylin and eosin. There was marked increase in weights in rats receiving high fat diet. Haematoxylin and eosin stained sections showed moderate fatty infiltration with empty spaces in hepatocytes with absent or pyknotic nuclei in high-fat group which were preserved to a great extent in group receiving fresh garlic along with high-fat diet. This study has proved that use of fresh garlic along with high-fat diet prevents its damaging effects in liver to a great extent

Effect of piroxicam and zinc on morphological and histochemical changes in mice liver

To observe the morphological and histochemical findings, produced by piroxicam and zinc in mice liver and correlate with serum hepatic enzyme. For this experimental study 30 adult mice [25 -30 grams] were obtained from animal house of Jinnah Postgraduate Medical Center, and divided into three groups i.e. A, B and C. Group A served as control and received normal diet, Group B received piroxicam 0.3 mg /Kg body weight intraperitoneally and Group C 1mg/Kg body weight of zinc intraperitoneally and piroxicam in the same dose as group B. After completion of study [6 weeks] animals were scarified and their livers were removed and after processing paraffin section were made and stained with Haematoxylin and Eosin for histological and histochemical examinations and correlate with serum hepatic enzyme level. Haematoxylin and Eosin stained section of group A indicated the normal histology and morphometry, the group B showed distorted hepatic lobular architecture. Central vein and sinusoids was dilated and congested, kupfer cell prominent and pyknotic cells and mono nuclear infiltration were seen. Group C showed altered histological findings comparable to group A. The Histochemical findings of group B showed depletion in glycogen content marked fibrosis of reticulin fibres and increased deposition of calcium phosphate crystals. In group C effect of zinc improve glycogen content and reticulin fiber deposition and decreased deposition of alkaline phosphatase crystals. The serum enzyme level of Alkaline Phosphatase and Serum Gulutamin Phosphatase significantly increased in group B animals and less significantly increase in group C as compared to group B animals. It was concluded that piroxicam in therapeutic dose, was toxic and produced hepatic injury and zinc along with piroxicam improved the hepatic damage

Protective role of zinc in piroxicam induced hepatic lobule damage in mice

To determine the effect of piroxicam in the hepatic lobule with protective role of zinc in mice and colorate the observation with serum enzyme level. An Experimental study. October 2008 to November 2008. Department of Anatomy, Basic Medical Sciences Institute, JPMC, Karachi. 30 adult mice [25-30gm] were divided into A, B and C groups. Group A served as a control, group B received piroxicam 0.3mg/kg body weight up, and group C received zinc 1mg/kg body weight lip and piroxicam as group B. After completion of study [i.e. 6 weeks] animals were scarified, blood sample was collected for serum enzyme level and their livers were removed and after processing, paraffin section were stained with H and E for the histological examination. U and E section of group A reveals normal histology of hepatic lobule, group B shows dilated central vein, irregular hepatic cord, distorted hepatocytes, dilated sinusoids and mononuclear infiltration, group C shows altered histological changes less then group B but more then group A Serum enzyme level [alkaline phosphatase] is within normal range in group A but significantly increase in group B and in group C less significantly increase as compare to group B but more then group A. Present study concludes that the therapeutic dose of piroxicam causes hepatic damage and zinc along with piroxicam is effective to prevent the hepatic injury